CD8A and neoplasm: This indicated that CD8+ T and Tregs showed high resemblance (high NES score) with tumor-associated T-cell signatures (CD8+ GZMK+ effector memory and CD4+ Tregs, respectively), whereas the CD4+ T populations resembled tumor/normal Th17 cells and naïve T-cell signatures (Supplementary Fig. S6C; ref. 26).