Since results from our mouse models indicate that the Sf3b1K700E mutation already plays a role during early stages of carcinogenesis (Figure 1G–L, Figure 1—figure supplement 1G), and the tumor-suppressing effect of TGF-β is most prominent on pre-cancerous epithelial cells (Massagué, 2008), we additionally established organoid lines with- and without Sf3b1K700E from non-cancerous mouse pancreata. This evidence concerns the gene TGFB1 and neoplasm.