The main purpose of this work was to delineate expression levels of AKR1B10/ITGA5 and the specific mechanism of AKR1B10/ITGA5 in GC cell proliferation, migration, invasion, and cell adhesion through in vitro molecular and cellular assays, thereby assisting in finding new molecular targets for GC diagnosis and treatment. Here, AKR1B10 is linked to gastric cancer.