In conclusion, PPARγ is required for mature adipocytes or obese mice (Imai et al., 2004), but at the same time, our results show that PPARγ expression does not differ by degree of obesity in adulthood and that obesity due to VitD3 is not achieved by affecting PPARγ expression. This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.