TNF and Huntington disease: Accordingly, using a multiplex bead-based assay (Figure 1H) and intracellular flow cytometry staining of TNF-α and IL-1β, we showed in monocytes purified from HD that CB inhibited the production of TNF-α and IL-1β (IC50 = 2,8 μM and IC50 = 8 μM, respectively) with no observed toxicity (Figure 1I).