Mouse macrophages, internalizing CML-EVs, display expression changes in genes associated with macrophage polarization such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) that are markedly upregulated, while nitric oxide synthase (iNOS) expression decreased, in line with M2 tumor-associated macrophage characteristics (72). Here, TNF is linked to neoplasm.