We further analyzed the relationship between AP4M1 gene alterations and HCC prognosis, and Kaplan-Meier plots and log-rank tests showed significant differences in OS (p = 2.486 × 10^-3) (Fig. 4B) and DFS (p = 6.955 × 10^-3) (Fig. 4C) between patients with and without gene alterations. The gene discussed is AP4M1; the disease is hepatocellular carcinoma.