As shown in Fig. 3I, we found that the lipid levels in tumour tissues were significantly higher than those in paired para-tumour tissues, which was consistent with the expression of SKA3 shown in Fig. 1E. Subsequently, we performed RT‒qPCR to examine the mRNA levels of ACLY, FASN, ACACA, and SCD in 70 CCA tissue samples. The gene discussed is ACACA; the disease is cholangiocarcinoma.