The results showed that Smad2 + 3, Smad4, Bcl2, and Caspase3 expression was changed in SHH medulloblastoma cell lines DAOY regardless of overexpression or knockdown of VASH2, indicating that VASH2 expression in myeloblasts may be mediated by affecting downstream indicators of TGFβ pathway. This evidence concerns the gene TGFB1 and medulloblastoma.