We found for the first time that the positive expression rate of VASH2 was closely associated with SHH-type pediatric medulloblastoma and that VASH2 was involved in the invasive, migratory, cell cycle, and apoptotic abilities of SHH medulloblastoma cell lines DAOY by affecting downstream indicators of the TGF-β pathway.VASH2 is expected to be a diagnostic and therapeutic target. This evidence concerns the gene VASH2 and medulloblastoma.