Furthermore, IHC analysis of tumors derived from these mice revealed that GFP-Bro40 treatment downregulated EGFR, p-EGFR, c-MET and their downstream effectors p-Akt and p-ERK in tumor tissues, which were accompanied by a decreased tumor proliferation rate (determined by Ki67 staining) and an increased apoptosis (Supplementary Fig. S6E). The gene discussed is AKT1; the disease is neoplasm.