After immunostaining for nuclei, Tyrosine Hydroxylase (TH, a marker for dopaminergic neurons) and alpha-synuclein (SNCA, a protein that accumulates in Lewy bodies and Lewy neurites in Parkinson’s disease and other synucleinopathies), we acquired images of isogenic dopaminergic neurons with and without the G2019S mutation. Here, SNCA is linked to Parkinson disease.