TNF and Hepatic steatosis: Also, HFHC-fed LV-Trim26 mice presented less liver steatosis than controls, as confirmed by the H&E staining, F4/80 staining, reduced NAS score, downregulated pro-inflammatory cytokines (e.g., IL-6, TNF-α, IL-1β, IL-18 and CCL2), and upregulated anti-inflammatory factor (i.e., IL-10) (P < 0.05 by 2 tailed t test; Supplementary Fig. S12e, f).