Considering the close relationship between CEBPD deactivation and catalysis function-associated RING domain activity of TRIM26 (including 2 conserved motifs), to further confirm whether TRIM26 with RING domain mutants are required for the inhibitory role of TRIM26 against NASH progression, we then subjected AAV-TBG-Trim26, AAV-TBG-Trim26 (△RING), AAV-TBG-Trim26 (AXXA) or AAV-TBG-Trim26 (AXXXXA) to 16-weeks WTDF-triggered NASH model in vivo (Supplementary Fig. 16a and Supplementary Fig. 17a). Here, CEBPD is linked to metabolic dysfunction-associated steatohepatitis.