For example, the mTORC1 pharmacological activator (MHY1485) induced the increase of total cellular O-GlcNAcylation and OGT levels in colon cell lines, while mTORC1 inhibition by rapamycin had the opposite effect, reducing total cellular O-GlcNAcylation and OGT levels in colon cells and various breast cancer cell lines (46, 65). This evidence concerns the gene OGT and breast cancer.