WD-fed Ldlr-/- mice become obese and develop multiple markers of NAFLD including insulin resistance (HOMA-IR), dyslipidemia, endotoxemia and elevated markers of hepatic macrosteatosis, inflammation, fibrosis as well as evidence of hepatic injury [alanine aminotransferase (ALT) and aspartate aminotransferase (AST)]. This evidence concerns the gene GPT and Insulin resistance.