PRF1 and infection: The results showed that at the early stage of infection, the accumulation of H2O2 in the stem of a sugarcane seedling inoculated with the ssccp1Δ (mixture of ssccp1Δ-1 × ssccp1Δ-2) and ssccp1Δ/con-PRF1 (mixture of ssccp1Δ/con-PRF1-1 × ssccp1Δ/con-PRF1-1) mutants exhibited a larger zone than those infected with the wild-type (mixture of MAT-1 × MAT-2) and ssccp1Δ/CCP1 (mixture of ssccp1Δ/CCP1−1 × ssccp2Δ/CCP1-2) strains (Fig. 6A).