In addition, in the 7-step CIC, cluster 2 exhibited a higher activation level in the main anti-tumor immune steps, including step 1 (release of cancer cell antigens), step 4 (recruitment of immune cells such as T cell, CD8 T, macrophage, NK cell, dendritic cell), step 6 (recognition of cancer cells by T cells) and step 7 (killing of cancer cells) (Figure 1D; Supplementary Table S6). The gene discussed is CD8A; the disease is neoplasm.