Thirdly, characterization of the mRNA expression levels of 13 target genes in 2 ESCC cell lines (KYSE140, KYSE150) showed that among CD44, GPC3, MET, EGFR, PSCA, MUC16 and CD22, at least one of the genes was relatively highly expressed in each ESCC cell line (Fig. 1C). This evidence concerns the gene CD22 and esophageal squamous cell carcinoma.