Through correlation analysis between METTL16 and the identified transcription factors in CCA tissues from the TCGA database, we found that 5 of the TFs were positively correlated with METTL16 in CCA tissues, including SP1, SP3, TBL1XR1, YY1, and ZBTB3 (Fig. 8B). This evidence concerns the gene SP1 and cholangiocarcinoma.