In parallel, we had employed a separate CCA xenograft model in which human CCA cells were inoculated into the livers of NOD/SCID mice; we observed that deletion of METTL16 by CRISPR/Cas9 also significantly inhibited CCA growth when the tumor cells were inoculated into the livers (Fig. 3G-I). Here, METTL16 is linked to cholangiocarcinoma.