The investigation of potential communication pathways between AML cells and different BM niche subpopulations, using the Multiplex assay, showed the following changes in the cytokine flux normalized to the levels measured in corresponding cell fractions derived from HDs: a significant reduction of IFNγ (0.7-fold, p = 0.0002) and IL-8 (0.7-fold, p = 0.012) levels along with a significant increase of SDF1-alpha (1.34-fold, p = 0.04) levels in AML cells co-cultured with non-induced AML-MSCs compared to non-induced HD-MSCs. This evidence concerns the gene CXCL8 and acute myeloid leukemia.