The potential of GUCA2A and COL3A1 expression level as a diagnostic biological parameter to distinguish CRC patients from healthy controls was demonstrated by ROC curve assessment, which was utilized to determine the sensitivity and specificity of GUCA2A expression (AUC 0.9773, 95% CI 0.9430 to 1.000, p-value < 0.0001) (Fig. 7A) and COL3A1 expression (AUC 0.9481, 95% CI 0.9017 to 0.9946, p-value < 0.0001) (Fig. 7B) for CRC diagnosis. The gene discussed is COL3A1; the disease is colorectal carcinoma.