We have further evaluated the concept that heritable transcriptional effects could occur in tissues beyond the liver, and identified minimal, but plausible transgenerational kidney transcriptional variation following CCl4-induced liver fibrosis, including at Zbtb16. Thus, effects transmitted through the germline may affect the transcriptome of multiple tissues, although the biological significance of the apparent transcriptional transmission and uncertainty on underlying mechanisms limit definitive conclusions. Here, ZBTB16 is linked to Hepatic fibrosis.