The cell viability was measured by CCK-8 assay and results showed that POLRMT silencing (by shPOLRMT-S1/S2, see Fig. 4) or KO (by CRISPR/Cas9 method, see Fig. 4) decreased viability (CCK-8 OD) in pCan1 primary cancer cells (Fig. 5A). This evidence concerns the gene POLRMT and cancer.