Immediately after stroke, mice were injected with either AAV5-EF1α-VEGF-P2A-eGFP (AAV-VEGF-eGFP), to induce VEGF and eGFP expression, or AAV5-EF1α-eGFP (AAV-eGFP), to induce only eGFP expression, into layer V of peri-infarct cortex (Fig. 7a–c). The gene discussed is VEGFA; the disease is Stroke.