An increase in the EphA2 level was induced by RNF5 shRNA in the three breast cancer cell lines, and the phosphorylation of EphA2 at S897 was decreased while that at Y772 was increased in RNF5 KD cells of all cell lines compared with the corresponding RNF5 WT cells (Fig. 3B, top). This evidence concerns the gene RNF5 and breast cancer.