AKT1 and breast cancer: To further investigate whether RNF5 commonly regulates EphA2 phosphorylation and downstream signaling across breast cancers, three other breast cancer cell lines, MDA-MB231, BT549 and BT474, were transfected with scramble or RNF5 shRNA, and EphA2 phosphorylation, as well as ERK and Akt phosphorylation, was quantified.