VANGL2 and Meningomyelocele: In contrast, the c740C > T, p.(Thr247Met) variant, which affects a non-conserved residue between zebrafish and human (Supplementary Fig. 1F) was able to function similarly to WT VANGL2 in all the assays except rescue of spaw expression (Fig. 8C), thus it does not seem capable of causing meningomyelocele and is likely to be a benign variant in this developmental context, as supported by in silico prediction (Fig. 8A).