To determine whether these clusters have DSG-specific autoreactive T cells, we identified the TCR repertoire in DSG3-reactive, activation-induced marker+ CD4+ T cells in PBMCs from 1 patient with PV (Supplemental Figure 4G) and matched TCRβ clones from the result to the TCR repertoire from scTCR-Seq from the same patient. The gene discussed is DSG1; the disease is acquired polycythemia vera.