NF1 and RASopathy: Estimations of Sanger sequencing prices were performed for five genes most commonly associated with RASopathies (BRAF, NF1, PTPN11, RAF1, and SOS1), and three genes, TCOF1, CHD7, and NIBPL most commonly associated with Treacher Collins, CHARGE, and Cornelia de Lange syndromes, respectively.