This effect on spheroid size was further increased when ES-2-Rv-NICD3 were combined with WT ES-2 cells (3:1) which we hypothesise could be due to intercellular signaling as ES-2 highly express Notch co-activator ligand Jagged-1, shown to regulate Notch3 signaling in ovarian cancer [9]. This evidence concerns the gene JAG1 and ovarian cancer.