In the second step of our study, using a cytometric multiplexing assay, we investigate the circulating levels of 6 cytokines, mostly monokines (IL-6, IL-8, TNF-α, IL-1β) produced by monocytes and macrophages, but also pro-inflammatory lymphocyte T helper (Th)1 (IL12p70) and anti-inflammatory lymphocyte Th2 (IL-10) related cytokines in a subgroup of MS patients (n=55). This evidence concerns the gene TNF and myeloid sarcoma.