Moreover, spheroid assay results showed that the tumor spheroids stably expressing MAGEA2/MAGEA3/MAGEA10, but not the empty vector transfected cells derived organoids, were capable of growing in the presence of Gem (Fig. 2F), whereas silencing MAGEA2/MAGEA3/MAGEA10 inhibited the growth of Capan-1 and T3M4 derived spheroids under Gem treatment (Fig. 2G, Additional file 4: Fig. S2D). The gene discussed is MAGEA3; the disease is neoplasm.