After verifying the targeting relationship between miR-205-3p and GLO1, we again performed rescue experiments and verified that GLO1 overexpression rescued the tumour suppressive effect of miR-205-3p on bladder cancer.Overall, miR-205-3p inhibits the biological behaviour of bladder cancer cells by negatively regulating GLO1. Here, GLO1 is linked to urinary bladder carcinoma.