In summary, this research confirmed that miR-205-3p targeting inhibited GLO1 to mediated P38/ERK phosphorylation to suppress the biological behavior of bladder cancer cells.An in-depth study on the function of miR-205-3p may help to determine the therapeutic targets that are expected to improve the clinical prognosis of bladder cancer patients. The gene discussed is GLO1; the disease is urinary bladder cancer.