Therefore, we sequenced the transcriptome of bladder cancer cells overexpressing miR-205-3p, and the sequencing results indicated that GLO1 was significantly downregulated in the miR-205-3p mimics group, in which the expression of GLO1 gene was significantly reduced and the presence of miR-205-3p in the 3’UTR region of GLO1 in three online bioinformatics analyses of sites with the miR-205-3p base binding site. Here, GLO1 is linked to urinary bladder carcinoma.