We constructed a 3D-ACC-immune cell co-culture model using UM-HACC-2A (adenoid cystic carcinoma cells), (which are generated from human ACC tumour that demonstrated a circumscribed collection of myoepithelial cells with a distinctive cribriform pattern characteristics of ACC) that exhibits epithelial morphology and high expressions of epithelial markers (E-cadherin, EGFR, pan-cytokeratin) and myoepithelial markers (P63)5, along with another salivary gland cancer 3D-preclinical cellular model, from mucoepidermoid carcinoma (A-253) cells. The gene discussed is CDH1; the disease is adrenal cortex carcinoma.