To delve deeper into the intricate interplay between PRMT6 and STAT3 R729 methylation in the context of tumor metastasis, we engineered MDA-MB-468 cancer cells to stably express various combinations: STAT3 WT+ vector, STAT3 WT + PRMT6, STAT3 R729K+ vector, and STAT3 R729K + PRMT6 (Fig. 7A). Here, STAT3 is linked to cancer.