To provide a further understanding of the differences and the molecular mechanism between the HLA‐B35 molecules from the Px and PY groups, our study focused on determining the biophysical differences between one PY molecule (HLA‐B*35:01), one Px molecule (HLA‐B*35:03) as well as one unclassified HLA‐B35 molecule (HLA‐B*35:05), which was found to have a protective effect in HIV‐1 subtype AE infection.20 The gene discussed is HLA-B; the disease is acrodermatitis enteropathica.