Gene set enrichment analysis (GSEA) showed high expression LYRM4-AS1 connected with P53 signaling pathway, signaling by NOTCH, cell cycle, JAK_STAT signaling pathway, and single-sample Gene Set Enrichment Analysis (ssGSEA) analysis and Spearman correlation analysis also suggested high LYRM4-AS1 expression level was correlated with immune infiltration, as well as the immune checkpoints in glioma. This evidence concerns the gene LYRM4 and glioma.