For all-stage HCC diagnosis, the combination of AFP and EV-MALAT1 had AUC 0.91 (HCC vs. nontumor); EV-MALAT1 and EV-SNHG1 had AUC 0.88 (HCC vs. CH/LC); For Very early HCC (mUICC stage I) EVDLEU2 and EV-MALAT1, and EV-HOTTIP and EVMALAT1 combinations had best AUC of 0.92 (HCC vs. nontumor) whereas combination EV-MALAT1 and EV-SNHG1 had AUC 0.98 for (HCC vs. CH/LC). The gene discussed is MALAT1; the disease is laryngotracheoesophageal cleft.