Figure 4A shows that, after PMA/Ionomycin stimulation, CD8lo T cell populations from HD as well as from SARS-CoV-2 infected patients (MD and SD) exhibited a lower frequency of the effector cytokine-producing cells, TNF, IL-2, and IFN-γ, than CD8hi T cells. The significant differences in both populations from the groups evaluated were confirmed by statistical analysis (Figure 4A). We found that SD patients exhibited a higher frequency of CD8hi T cells expressing IL-2 compared to MD patients. We did not find differences in the expression of other cytokines such as TNF and INFγ (Figure 4A). This evidence concerns the gene TNF and Menkes disease.