After detecting changes in mitochondrial membrane potential, we found that the fluorescence of ccRCC cells in the sh-NR3C1 group changed from red to green, with a trend similar to that of the positive control group of carbonylcyanide-m- chlorophenylhydrazone (CCCP), indicating that knockdown of NR3C1 induced mitochondrial membrane potential damage in ccRCC cells (P < 0.001) (Fig. 6F–H). The gene discussed is NR3C1; the disease is nonpapillary renal cell carcinoma.