For instance, Rosenblum et al. applied novel amino-ionizable lipid L8-composed LNPs to intracerebrally deliver Cas9 endonuclease mRNA and sgRNA targeting polo-like kinase 1 (PLK1), a kinase necessary for mitosis, in aggressive orthotopic glioblastoma in vivo. The gene discussed is PLK1; the disease is glioblastoma.