ACTA1 and Hepatic fibrosis: To confirm this, we performed hematoxylin and eosin (H&E) staining, Masson staining, Sirius red staining, and immunohistochemistry (IHC) for α-SMA, COL1α1, and TGFβ, and the data revealed that lnc-Helf silencing alleviated hepatic fibrosis induced by CCl4 (Fig. 2F and Additional file 1: Fig. S3A).