These included age, sex (male), smoking, COPD, pre-stroke dependence (mRS ≥ 2), dysphagia, disturbance of consciousness, total muscle strength of the worse side, GCS (≤ 14), multilobar involvement, deep region involvement, extension into ventricles, lesion volume(≥ 20mL), albumin(< 40 g/L), blood glucose (> 6.1mmol/L) and creatinine(> 97μmol/L). The gene discussed is ALB; the disease is Stroke.