Given that the presence of Arg177 and Asp159 in dogs causes unique charge distribution patterns on the front and back sides of dog PrPC, which might in turn protect dogs from getting prion diseases (27, 28, 29, 52, 53), we predicted that the two residues in dog PrP might regulate the LLPS of human PrPC by increasing the saturation concentration for PrP condensation. Here, PRNP is linked to prion disease.