CD40LG and Lyme disease: It has been shown that enzyme immunoassays forthe detection of IgM more often lead to false positive results, whichmay be caused by their lower affinity.39−42 Detection of IgM to recognizethe early stage of Lyme disease is highly problematic and needs improvement.A study by Webber et al. (2019) estimated that false positives inIgM WB account for up to 53% of results, which causes overdiagnosisof Lyme diseases.