RB1 and neoplasm: For example, in analysis of a tumor nodule induced by SpCas9 together with 35 sgRNAs each at 0.8 mg, in addition to their respective wild-type (WT) allele, the p53 target site had 3 types of mutations, 28.12% for 1-bp deletion on the left side of the break (termed Del1|0), 16.13% for 7-bp deletion on the right side of the break (Del0|7), and 13.87% for insertion of 1A at the break (Ins1A); Atm had two (23.81% for D6|0 and 20.07% for Ins1C), and Rb1 had none in addition to WT alleles (Fig. 2A).