We expressed known misfolding-prone proteins, amyotrophic lateral sclerosis-linked G93A mutant of copper-zinc superoxide dismutase [SOD1(G93A)]31,55, synphilin1 (SYN1; which is enriched in cytosolic inclusion bodies)31,56, and the truncated form of glutathione peroxidase 1 (GPx1-Ter)16 in HeLa cells. Here, SYN1 is linked to amyotrophic lateral sclerosis.