The inhibition of TSC2 was found to activate mTORC1 signaling in GBM cells, and G6PD was considerably upregulated in cells transfected with the pLKO.1 plasmids targeting TSC2 compared to cells transfected with the pLKO.1 plasmid with a non-targeting shRNA sequence. Here, TSC2 is linked to glioblastoma.