To understand signalling pathways downstream of CaSR in AML LSC, we performed an RNA sequencing analysis of WT vs CaSR KO MLL-AF9+ Lin− cells, which revealed 4 up- and 48 downregulated DEG and downregulation of downstream target genes in the MAPK/ERK pathway in CaSR KO compared to WT cells (Fig. 4a). This evidence concerns the gene MLLT3 and acute myeloid leukemia.