To investigate whether PLCL1 promotes autophagic flux, RCC cells were treated with 3-MA or rapamycin, and western blotting was performed to determine the protein levels of microtubule-associated protein 1 light chain 3β (LC3B) and sequestosome 1 (SQSTM1 or p62). The gene discussed is PLCL1; the disease is renal cell carcinoma.