We also aimed to evaluate the hypercholesterolemia-induced alterations in ATP and NAD levels in the mBMECs, and changes in the energetic metabolism based on glycolytic function: glycolytic capacity, glycolytic reserve, and non-glycolytic acidification in mBMECs of LDLR−/−/Apo E−/− double knockout mice versus the control mice. The gene discussed is APOE; the disease is Hypercholesterolemia.