HFD also increased the overall number of ductal IBA1+ macrophages (Fig. 7A; P < 0.0001 LF NW vs HF NW; P = 0.0002 LF FW vs HF FW) independent of weaning status (Fig. 7B, P < 0.0001; Fig. 7C, P = NS). This evidence concerns the gene AIF1 and hydrops fetalis.