Considering the intersection with HFD, obesity can promote the accumulation of CCR7+ macrophages and dendritic cells in adipose tissue in close proximity to lymph nodes [25]; however, in this model we were not able to co-localize IBA1 and CCR7 (in two different staining panels) to assess this. This evidence concerns the gene AIF1 and obesity due to melanocortin 4 receptor deficiency.